2YXY
Crystarl structure of Hypothetical conserved protein, GK0453
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 120 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.117 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 75.689, 75.689, 64.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.950 - 2.200 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sf9 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.078 | 0.279 |
Number of reflections | 9952 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 10.3 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | oil-batch | 8.13 | 293 | 0.1M Tris-HCl, 13.3%(w/v) PEG MME2000, 0.01M NiCl, pH 8.13, oil-batch, temperature 293K |