2YXQ
The plug domain of the SecY protein stablizes the closed state of the translocation channel and maintains a membrane seal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2006-12-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97921 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.235, 148.489, 81.072 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.500 |
Rwork | 0.301 |
R-free | 0.31700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rhz |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | DM |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.087 | 0.787 |
Number of reflections | 14678 | |
<I/σ(I)> | 21.9 | 2.3 |
Completeness [%] | 98.6 | 97.5 |
Redundancy | 12 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 40-55% PEG400, 50mM Glycine-HCl , pH9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |