2YXM

Crystal structure of I-set domain of human Myosin Binding ProteinC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9790
Spacegroup name	P 41 21 2
Unit cell lengths	55.199, 55.199, 70.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.380 - 1.510
R-factor	0.196
Rwork	0.196
R-free	0.21200
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.510	1.510
Number of reflections	17469
<I/σ(I)>	78.6	5.9
Completeness [%]	98.7	90.4
Redundancy	26.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	293	0.2M pottasium sodium tartrate tetrahydrate, 0.1M tri-sodium citrate dihydrate, 2M ammonium sulfate, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K