2YXM
Crystal structure of I-set domain of human Myosin Binding ProteinC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.199, 55.199, 70.134 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.380 - 1.510 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.21200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Number of reflections | 17469 | |
| <I/σ(I)> | 78.6 | 5.9 |
| Completeness [%] | 98.7 | 90.4 |
| Redundancy | 26.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | 0.2M pottasium sodium tartrate tetrahydrate, 0.1M tri-sodium citrate dihydrate, 2M ammonium sulfate, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
