2YXG
Crystal structure of Dihyrodipicolinate Synthase (dapA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.469, 76.532, 101.863 |
| Unit cell angles | 90.00, 106.87, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.16115 |
| Rwork | 0.158 |
| R-free | 0.22444 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o5k |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 | 0.211 |
| Number of reflections | 58517 | |
| Completeness [%] | 97.6 | 82.6 |
| Redundancy | 3.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Oil-batch | 6.5 | 293 | 18% PEG 8000, 0.1M Cacodylate, 0.2M Ca Acetate, pH 6.5, Oil-batch, temperature 293.0K |
