2YXF
The high resolution crystal structure of beta2-microglobulin under physiological conditions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2005-12-05 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.65 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.644, 28.907, 54.397 |
Unit cell angles | 90.00, 121.59, 90.00 |
Refinement procedure
Resolution | 26.490 - 1.130 |
R-factor | 0.18436 |
Rwork | 0.184 |
R-free | 0.19486 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d4f |
RMSD bond length | 0.006 |
RMSD bond angle | 1.227 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.630 | 1.190 |
High resolution limit [Å] | 1.130 | 1.130 |
Rmerge | 0.051 | 0.332 |
Number of reflections | 38852 | |
<I/σ(I)> | 12.4 | 4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | 25% PEG 4000, 25% Glycerol, 0.06M ammoniumacetate, 0.1M MOPS, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 288K |