2YX1

Crystal structure of M.jannaschii tRNA m1G37 methyltransferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-05
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97934, 0.97942, 0.96418
Spacegroup name	P 1 21 1
Unit cell lengths	62.000, 69.790, 87.150
Unit cell angles	90.00, 96.07, 90.00

Refinement procedure

Resolution	47.900 - 2.200
R-factor	0.224
Rwork	0.224
R-free	0.27600
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.600
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge		0.504
Number of reflections	36694
<I/σ(I)>	21.9	1.8
Completeness [%]	96.8	72.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2M sodium cacodylate, 7.5% PEG 3350, 5% PEGMME 550, 0.02M Hepes pH 7.50, 0.002M zinc sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K