2YWW
Crystal structure of aspartate carbamoyltransferase regulatory chain from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-29 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.282150, 1.20000, 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.003, 71.398, 92.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.960 - 2.000 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 3.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.211 |
| Number of reflections | 23638 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.16M Ammmounium Acetate, 0.08M Bis-Tris(pH5.5), 20%w/v PEG3350, 10mM ATP, 10mM Zinc chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
