2YWV
Crystal structure of SAICAR synthetase from Geobacillus kaustophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-05 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.890, 56.397, 56.410 |
| Unit cell angles | 76.39, 64.36, 64.35 |
Refinement procedure
| Resolution | 37.000 - 1.750 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gqs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.038 | 0.118 |
| Number of reflections | 92845 | |
| Completeness [%] | 93.9 | 94.3 |
| Redundancy | 1.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.2M Ammonium Sulfate, 0.1M Sodium Acetate trihydrate(pH4.6), 30%w/v PEG MME 2000 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
