2YW8
Crystal structure of human RUN and FYVE domain-containing protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.2752 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 115.150, 115.150, 115.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.010 - 3.000 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vfy |
RMSD bond length | 0.011 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 5650 | |
<I/σ(I)> | 30.2 | 12.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 40.9 | 42.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Bis-tris, Ammonium sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |