2YVZ

Crystal structure of magnesium transporter MgtE cytosolic domain, Mg2+-free form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97909, 0.97926, 0.96403
Spacegroup name	P 21 21 21
Unit cell lengths	77.112, 100.183, 100.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.800 - 3.900
R-factor	0.346
Rwork	0.346
R-free	0.37000
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.970
High resolution limit [Å]	3.900	3.900
Number of reflections	7435
<I/σ(I)>	11.6	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2M ammonium acetate, 0.5% n-octyl-beta-D-glucopyranoside, 10% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K