2YVX
Crystal structure of magnesium transporter MgtE
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97924, 0.97933, 0.98200 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 118.314, 134.897, 366.252 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.690 - 3.500 |
R-factor | 0.294 |
Rwork | 0.294 |
R-free | 0.34100 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 37118 | |
<I/σ(I)> | 8.6 | 2.3 |
Redundancy | 16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 2-methyl-2,4-pentanediol (MPD), 40mM magnesium acetate, 100mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |