2YVV
Crystal structure of hyluranidase complexed with lactose at 2.6 A resolution reveals three specific sugar recognition sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 203 |
| Detector technology | CCD |
| Collection date | 2006-12-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.803 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 59.061, 59.061, 588.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.19345 |
| Rwork | 0.191 |
| R-free | 0.22601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dp5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.626 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 13012 | |
| <I/σ(I)> | 6.7 | 2.3 |
| Completeness [%] | 99.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | TRIS HCL, SODIUM FORMATE, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
