2YVS
Crystal structure of glycolate oxidase subunit GlcE from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-13 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.880, 71.690, 89.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.547 - 2.000 |
Rwork | 0.208 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SeMet crystal of Glycolate oxidase subunit GlcE |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.989 |
High resolution limit [Å] | 2.000 |
Number of reflections | 30844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1M Na3 Citrate, 2.2M Na Formate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |