2YVR
Crystal structure of MS1043
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.2820, 1.2831 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 38.950, 38.950, 125.835 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.120 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.21800 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 9725 | |
| <I/σ(I)> | 29.1 | 10.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 14.6 | 15.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | HEPES, PEG4000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
