2YVJ
Crystal structure of the ferredoxin-ferredoxin reductase (BPHA3-BPHA4)complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-12 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.596, 173.715, 60.976 |
Unit cell angles | 90.00, 115.81, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.1789 |
R-free | 0.26150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.024 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.082 | 0.484 |
Number of reflections | 86196 | |
<I/σ(I)> | 21.18 | 5.46 |
Completeness [%] | 96.7 | 93.3 |
Redundancy | 9.3 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES, 30%(W/V) PEG4000, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K |