2YVG
crystal structure of ferredoxin reductase, BPHA4 (blue-semiquinone)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-30 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 98.288, 98.288, 170.066 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.600 |
R-factor | 0.195 |
Rwork | 0.194 |
R-free | 0.22000 |
Structure solution method | DIFFERENCE FOURIER |
RMSD bond length | 0.012 |
RMSD bond angle | 1.468 |
Data scaling software | XDS |
Refinement software | XTALVIEW |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.680 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.054 | 0.641 |
Number of reflections | 63621 | |
<I/σ(I)> | 36.5 | 5.3 |
Completeness [%] | 98.6 | 97.7 |
Redundancy | 20.8 | 18.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | 2M SODIUM FORMATE, 0.1M SODIUM ACETATE, pH 5.40, VAPOR DIFFUSION, SITTING DROP, temperature 293K |