2YVC
Crystal structure of the Radixin FERM domain complexed with the NEP cytoplasmic tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 108.790, 116.838, 141.884 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.710 - 3.200 |
R-factor | 0.2344 |
Rwork | 0.233 |
R-free | 0.26712 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gc7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.203 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.710 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 30656 | |
Completeness [%] | 99.7 | 97.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 20% PEG3350, 0.2M DL-maleic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |