2YV9
Crystal structure of the CLIC homologue EXC-4 from c. elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2004-08-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.913, 91.395, 63.286 |
| Unit cell angles | 90.00, 99.61, 90.00 |
Refinement procedure
| Resolution | 16.610 - 1.600 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k0m |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.456 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.630 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.520 |
| Number of reflections | 61432 | |
| <I/σ(I)> | 16.7 | 2.5 |
| Completeness [%] | 75.8 | 75.8 |
| Redundancy | 3.65 | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG 3350, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
