2YV8
Crystal structure of N-terminal domain of human galectin-8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-22 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9792, 0.9797, 0.964 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.668, 50.012, 69.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.570 - 1.920 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.076 | |
| Number of reflections | 26234 | |
| <I/σ(I)> | 60 | 29.2 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Magnesium formate, 15% PEG 3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
