2YV4
Crystal Structure of C-terminal Sua5 Domain from Pyrococcus horikoshii Hypothetical Sua5 Protein PH0435
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-21 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97886, 0.90, 0.97929 |
| Spacegroup name | P 61 |
| Unit cell lengths | 36.144, 36.144, 135.867 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.300 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.200 |
| Number of reflections | 6812 | |
| <I/σ(I)> | 47.8 | 13.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.6 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium sulfate, 30% w/v PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
