2YRX
Crystal structure of GAR synthetase from Geobacillus kaustophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-23 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.792, 83.362, 96.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.230 - 1.900 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yrw |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.102 | 0.655 |
Number of reflections | 32811 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 10.3 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.2M ammonium sulfate, 0.1M sodium acetate trihydrate, 30% polyethylene glycol monomethyl ether 2000, 10mM ATP, pH4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |