2YR6
Crystal structure of L-phenylalanine oxidase from Psuedomonas sp.P501
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.877, 113.029, 136.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.180 - 1.350 |
| R-factor | 0.10439 |
| Rwork | 0.103 |
| R-free | 0.13769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yr4 |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.475 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.180 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.089 | 0.342 |
| Number of reflections | 339311 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1M HEPES pH7.5, 1.0M ammonium sulfate, VAPOR DIFFUSION, temperature 293K |
