2YR4
Crystal structure of L-phenylalanine oxiase from Psuedomonas sp. P-501
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 141.577, 145.405, 82.076 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.770 - 1.700 |
R-factor | 0.1675 |
Rwork | 0.166 |
R-free | 0.19525 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.599 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.770 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.081 | 0.272 |
Number of reflections | 184918 | |
Completeness [%] | 99.4 | 96.9 |
Redundancy | 6.5 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1M HEPES pH 7.5, 1.7M ammonium sulfate, VAPOR DIFFUSION, temperature 293K |