2YR0
Crystal Structure of Hypothetical Methyltransferase TTHA0223 from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-09 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9792, 0.9000, 0.9797 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.901, 43.556, 84.761 |
| Unit cell angles | 90.43, 102.36, 97.05 |
Refinement procedure
| Resolution | 41.370 - 1.900 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yqz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.420 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.264 |
| Number of reflections | 37923 | |
| <I/σ(I)> | 8.9 | 2.3 |
| Completeness [%] | 96.3 | 96.9 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | 0.2M CaCl2, 20%(w/v) PEG 3350, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
