2YQY
Crystal structure of TT2238, a four-helix bundle protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-16 |
Detector | RIGAKU |
Wavelength(s) | 0.97914, 0.97950, 0.90000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 104.846, 104.846, 51.254 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.980 - 2.000 |
R-factor | 0.22208 |
Rwork | 0.220 |
R-free | 0.25738 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.375 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.058 | 0.142 |
Number of reflections | 19771 | |
<I/σ(I)> | 22.4 | 8.5 |
Completeness [%] | 99.6 | 100 |
Redundancy | 13.8 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 291 | 20%(w/v) PEG 1000, 100mM Tris pH 7.0, 0.2M Zn acetate, microbatch, temperature 291K |