2YQU
Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(E3s) from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-29 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.411, 105.760, 84.907 |
| Unit cell angles | 90.00, 92.65, 90.00 |
Refinement procedure
| Resolution | 32.620 - 1.700 |
| R-factor | 0.19853 |
| Rwork | 0.195 |
| R-free | 0.22988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JEH (CRYSTAL STRUCTURE OF YEAST E3 LIPOAMIDE DEHYDROGENASE) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.104 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.700 |
| Rmerge | 0.037 | 0.194 |
| Number of reflections | 104936 | |
| <I/σ(I)> | 12.7 | 2.3 |
| Completeness [%] | 98.6 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
