2YQU
Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(E3s) from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-29 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.411, 105.760, 84.907 |
Unit cell angles | 90.00, 92.65, 90.00 |
Refinement procedure
Resolution | 32.620 - 1.700 |
R-factor | 0.19853 |
Rwork | 0.195 |
R-free | 0.22988 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1JEH (CRYSTAL STRUCTURE OF YEAST E3 LIPOAMIDE DEHYDROGENASE) |
RMSD bond length | 0.007 |
RMSD bond angle | 1.104 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.670 | 1.700 |
Rmerge | 0.037 | 0.194 |
Number of reflections | 104936 | |
<I/σ(I)> | 12.7 | 2.3 |
Completeness [%] | 98.6 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |