2YQ8
Crystal structure of the SeMet-labeled N-terminal domain and peptide substrate binding domain of alpha subunit of prolyl-4 hydroxylase type I from human.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-28 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 104.878, 72.017, 66.431 |
Unit cell angles | 90.00, 91.67, 90.00 |
Refinement procedure
Resolution | 44.622 - 2.987 |
R-factor | 0.2124 |
Rwork | 0.209 |
R-free | 0.27740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v5f |
RMSD bond length | 0.012 |
RMSD bond angle | 1.502 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 3.070 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.080 | 0.330 |
Number of reflections | 19064 | |
<I/σ(I)> | 13.23 | 2.9 |
Completeness [%] | 96.7 | 73.9 |
Redundancy | 3.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 26-29% PEGMME 2000, 200MM KSCN AND 5MM DTT, 5MM SPERMINE TETRAHYDROCHLORIDE, pH 8.5 |