2YQ1
MHV-68 LANA (ORF73) C-terminal domain: triclinic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-05 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 35.958, 53.395, 70.859 |
Unit cell angles | 106.00, 93.47, 109.80 |
Refinement procedure
Resolution | 24.062 - 2.300 |
R-factor | 0.2024 |
Rwork | 0.200 |
R-free | 0.24560 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.009 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.270 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.100 | 0.380 |
Number of reflections | 24125 | |
<I/σ(I)> | 7.4 | 2.34 |
Completeness [%] | 92.8 | 80.8 |
Redundancy | 2.03 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.5 UL OF 0.3 MM PROTEIN IN 5 MM BISTRIS, PH 6.5, 5 MM DTT WERE ADDED TO 1.5 UL OF 2.0 M SODIUM FORMATE, 15% (W/V) PEG 6000. THE MIXTURE WAS INCUBATED AT 20 DEGREE CENTIGRADE IN A HANGING DROP SETUP. CRYSTALS GREW IN A FEW DAYS AND WERE CRYO-PROTECTED BY SHORT SOAKING IN 2.0 M SODIUM FORMATE, 15% (W/V) PEG 6000, 25% (V/V) GLYCEROL. |