2YQ0
KSHV LANA (ORF73) C-terminal domain, octameric ring: cubic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-05 |
Detector | MARRESEARCH |
Spacegroup name | P 4 3 2 |
Unit cell lengths | 107.400, 107.400, 107.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.963 - 3.911 |
R-factor | 0.2623 |
Rwork | 0.260 |
R-free | 0.31660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ypy |
RMSD bond length | 0.003 |
RMSD bond angle | 0.746 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.010 |
High resolution limit [Å] | 3.910 | 3.910 |
Rmerge | 0.110 | 0.730 |
Number of reflections | 2178 | |
<I/σ(I)> | 21.73 | 3.92 |
Completeness [%] | 99.7 | 100 |
Redundancy | 10.8 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 2.5 | 277 | 1 UL OF 1 MM PROTEIN IN 5 MM BISTRIS, PH 6.5, 2 MM DTT WAS ADDED TO 1 UL OF 0.1 M SODIUM CITRATE, PH 2.5, 1.5 M AMMONIUM SULFATE. THE MIXTURE WAS INCUBATED AT 4 DEGREE CENTIGRADE IN A HANGING DROP SETUP. CRYSTALS GREW IN A FEW DAYS AND WERE CRYO-PROTECTED BY SHORT SOAKING IN 0.1 M SODIUM CITRATE, PH 2.5, 1.5 M AMMONIUM SULFATE, 25% (V/V) GLYCEROL. |