2YPK
Structural features underlying T-cell receptor sensitivity to concealed MHC class I micropolymorphisms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.829, 82.067, 110.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.840 - 1.950 |
| R-factor | 0.19427 |
| Rwork | 0.191 |
| R-free | 0.25182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bvo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.539 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.050 | 0.310 |
| Number of reflections | 34422 | |
| <I/σ(I)> | 19.4 | 3.4 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 2.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 16% PEG 8000, 50MM MES PH6.5 |
