2YPJ
Non-catalytic carbohydrate binding module CBM65B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-14 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.920, 57.920, 116.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.880 - 2.350 |
R-factor | 0.22706 |
Rwork | 0.224 |
R-free | 0.28035 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ba6 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.556 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.370 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.080 | 0.660 |
Number of reflections | 8856 | |
<I/σ(I)> | 13.4 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.2 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 200 AMMONIUM ACETATE, 100 MM TRI-SODIUM CITRATE PH 5.6, 30% PEG 4000 |