2YPH
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, with mutation H230S, crystallized with 2',3-(RP)- cyclic-AMPS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-02 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.630, 47.530, 107.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.887 - 2.100 |
R-factor | 0.2329 |
Rwork | 0.230 |
R-free | 0.28880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xmi |
RMSD bond length | 0.002 |
RMSD bond angle | 0.502 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.090 | 0.840 |
Number of reflections | 12336 | |
<I/σ(I)> | 13.4 | 2 |
Completeness [%] | 96.7 | 92.1 |
Redundancy | 5.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | 277 | 250 UM PROTEIN AND 10 MM 23-(RP)-CYCLIC AMPS WAS MIXED IN 0.5 PLUS 0.5 UL DROPS WITH 30% PEG4000 AND 50 MM ACETATE (2:1 PH 3 AND 5) IN 4C TEMP |