2YOQ
Structure of FAM3B PANDER E30 construct
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-11 |
Detector | ADSC CCD |
Spacegroup name | P 31 |
Unit cell lengths | 86.540, 86.540, 96.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.640 - 2.350 |
R-factor | 0.1599 |
Rwork | 0.158 |
R-free | 0.18970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.160 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.470 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.130 | 0.790 |
Number of reflections | 33513 | |
<I/σ(I)> | 11.2 | 2.6 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 4.3 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |