2YOI
Crystal Structure of Ancestral Thioredoxin Relative to Last Eukaryotes Common Ancestor (LECA) from the Precambrian Period
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.366, 47.772, 73.836 |
| Unit cell angles | 90.00, 98.49, 90.00 |
Refinement procedure
| Resolution | 25.583 - 1.300 |
| R-factor | 0.1575 |
| Rwork | 0.156 |
| R-free | 0.18440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2trx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.600 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.050 | 0.070 |
| Number of reflections | 46574 | |
| <I/σ(I)> | 18.4 | 7.9 |
| Completeness [%] | 94.4 | 89.8 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | VAPOUR DIFFUSION, SITTING DROP; 1:1 RATIO MAGNESIUM ACETATE TETRAHYDRATE, 20% PEG 3350 T 293 K, pH 5.5 |
