2YOA
Synaptotagmin-1 C2B domain with phosphoserine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 97.000, 97.000, 89.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.500 - 1.500 |
| R-factor | 0.18361 |
| Rwork | 0.183 |
| R-free | 0.20404 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uov |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.260 |
| Number of reflections | 66840 | |
| <I/σ(I)> | 43.9 | 7.9 |
| Completeness [%] | 97.7 | 81.7 |
| Redundancy | 12.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M KSCN, 20 % PEG 600 |
