2YO3
Salmonella enterica SadA 1185-1386 fused to GCN4 adaptors (SadAK14)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-29 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.320, 46.170, 103.760 |
| Unit cell angles | 90.00, 98.03, 90.00 |
Refinement procedure
| Resolution | 37.310 - 2.000 |
| R-factor | 0.20238 |
| Rwork | 0.200 |
| R-free | 0.25292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.099 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.610 |
| Number of reflections | 59695 | |
| <I/σ(I)> | 12.4 | 2.32 |
| Completeness [%] | 98.3 | 96.8 |
| Redundancy | 3.91 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% (W/V) PEG 8000, 100 MM MAGNESIUM ACETATE, 100 MM TRIS PH 8.5 |
