2YO2
Salmonella enterica SadA 255-358 fused to GCN4 adaptors (SadAK12)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 48.630, 48.630, 366.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.260 - 2.000 |
| R-factor | 0.24413 |
| Rwork | 0.242 |
| R-free | 0.28374 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.294 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.640 |
| Number of reflections | 18662 | |
| <I/σ(I)> | 13.8 | 2.29 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 7.3 | 6.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 500 MM AMMONIUM TARTRATE, 100 MM SODIUM ACETATE PH 5.0 |
