2YNS
rImp_alpha_B54NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.242, 141.405, 73.325 |
| Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
| Resolution | 19.908 - 2.100 |
| R-factor | 0.1748 |
| Rwork | 0.177 |
| R-free | 0.19740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b8p |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.681 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.910 |
| Number of reflections | 73652 | |
| <I/σ(I)> | 10.8 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRISPROPANE PH 7.0, 15-17% PEG 3350 AND 0.2 M NAF |
