2YNP
yeast betaprime COP 1-604 with KTKTN motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2011-07-02 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 31 |
| Unit cell lengths | 127.250, 127.250, 59.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.625 - 2.962 |
| R-factor | 0.2425 |
| Rwork | 0.240 |
| R-free | 0.29430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2YNO 3mkq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.381 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.600 | 3.040 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 22113 | |
| <I/σ(I)> | 15.8 | 2 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BICINE PH 9.0, 10% MPD |
