2YNO
yeast betaprime COP 1-304H6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-05-25 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.560, 50.510, 74.470 |
| Unit cell angles | 90.00, 103.99, 90.00 |
Refinement procedure
| Resolution | 49.015 - 1.800 |
| R-factor | 0.1898 |
| Rwork | 0.188 |
| R-free | 0.22330 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.220 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.100 |
| Number of reflections | 55618 | |
| <I/σ(I)> | 24 | 10.2 |
| Completeness [%] | 96.6 | 81.6 |
| Redundancy | 3.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG1500 |
