2YNN
yeast betaprime COP 1-304 with KTKTN motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2011-07-02 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 61 |
| Unit cell lengths | 129.160, 129.160, 60.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.928 - 1.782 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.17990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yno |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.206 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 1.770 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.130 | 0.660 |
| Number of reflections | 54637 | |
| <I/σ(I)> | 8.7 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M HEPES PH 7.5, 0.5M AMMONIUM SULFATE, 30% V/V MPD |
