2YNM
Structure of the ADPxAlF3-Stabilized Transition State of the Nitrogenase-like Dark-Operative Protochlorophyllide Oxidoreductase Complex from Prochlorococcus marinus with Its Substrate Protochlorophyllide a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-17 |
Detector | RAYONIX |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 308.400, 74.110, 74.230 |
Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
Resolution | 34.510 - 2.100 |
R-factor | 0.2006 |
Rwork | 0.199 |
R-free | 0.23310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3FWY AND 2XDQ |
RMSD bond length | 0.004 |
RMSD bond angle | 1.018 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.510 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.050 | 0.260 |
Number of reflections | 81370 | |
<I/σ(I)> | 14.32 | 3 |
Completeness [%] | 83.1 | 48.2 |
Redundancy | 2.6 | 0.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | CRYSTALLIZED FROM 3% (W/V) PEG 6000, 100MM KCL, 100MM TRIS, PH 8.5; THEN SOAKED WITH 13% (W/V) PEG 8000, 100MM HEPES, PH 7.5, 15% (V/V) GLYCEROL, 5MM DTT, 0.01MM PCHLIDE, 24MM DMSO |