2YNK

Wzi, an Outer Membrane Protein Involved in Group 1 Capsule Assembly in Escherichia coli, is a Carbohydrate Binding Beta-Barrel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-31
Detector	MARRESEARCH
Spacegroup name	C 2 2 2
Unit cell lengths	136.660, 152.080, 95.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.370 - 2.640
R-factor	0.24656
Rwork	0.245
R-free	0.27905
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	1.163
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCDE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.370	2.740
High resolution limit [Å]	2.640	2.640
Rmerge	0.080	0.750
Number of reflections	28226
<I/σ(I)>	16.9	2.9
Completeness [%]	99.2	98.8
Redundancy	10	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				SEE PAPER