2YNH
HIV-1 Reverse Transcriptase in complex with inhibitor GSK500
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-13 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 118.987, 154.567, 156.755 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.400 - 2.900 |
R-factor | 0.20848 |
Rwork | 0.207 |
R-free | 0.26240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yng |
RMSD bond length | 0.007 |
RMSD bond angle | 1.114 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.090 | 0.460 |
Number of reflections | 32529 | |
<I/σ(I)> | 21.8 | 3.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.4 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 100MM HEPES PH 7.5, 10MM SPERMIDINE, 1.1M SODIUM POTASSIUM TARTRATE |