2YNF
HIV-1 Reverse Transcriptase Y188L mutant in complex with inhibitor GSK560
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.578, 154.508, 157.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.570 - 2.360 |
| R-factor | 0.1984 |
| Rwork | 0.197 |
| R-free | 0.23631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hmv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.354 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.060 | 0.450 |
| Number of reflections | 59250 | |
| <I/σ(I)> | 23.4 | 4.15 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100MM HEPES PH7.5, 10MM SPERMIDINE, 1.1M SODIUM POTASSIUM TARTRATE |
