2YN7
Crystal structure of an outer surface protein BBA66 from Borrelia burgdorferi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-01 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 |
Unit cell lengths | 40.910, 51.120, 58.880 |
Unit cell angles | 90.34, 96.21, 94.40 |
Refinement procedure
Resolution | 29.780 - 2.250 |
R-factor | 0.19032 |
Rwork | 0.188 |
R-free | 0.23232 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4axz |
RMSD bond length | 0.012 |
RMSD bond angle | 1.540 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.010 | 2.360 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.060 | 0.290 |
Number of reflections | 21363 | |
<I/σ(I)> | 9.7 | 2.2 |
Completeness [%] | 94.3 | 85.2 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 18% PEG 2000 MME, 0.1 M TRIS PH 9.0 |