2YN6
Pentameric Ligand-Gated Ion Channel ELIC in Complex with Barium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-30 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 43 |
Unit cell lengths | 100.473, 100.473, 263.951 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.986 - 3.310 |
R-factor | 0.2285 |
Rwork | 0.227 |
R-free | 0.25780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vl0 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.218 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.500 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.120 | 0.640 |
Number of reflections | 38612 | |
<I/σ(I)> | 12.1 | 2.7 |
Completeness [%] | 99.4 | 96.6 |
Redundancy | 6.5 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 50 MM ADA PH6.5, 50 MM BAAC2, 10% PEG4000 |