2YN5
Structural insight into the giant calcium-binding adhesin SiiE: implications for the adhesion of Salmonella enterica to polarized epithelial cells
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.400, 69.040, 67.260 |
| Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
| Resolution | 47.000 - 1.850 |
| R-factor | 0.20927 |
| Rwork | 0.206 |
| R-free | 0.24626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yn3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.431 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.540 |
| Number of reflections | 50496 | |
| <I/σ(I)> | 13.9 | 3.2 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 0.1 M SODIUM CACODYLATE, PH 6.7, 0.2 M CALCIUM ACETATE, 16 % W/V PEG 8000, 3% 6-AMINOHEXANOIC ACID |
