2YMT

gamma 2 adaptin EAR domain crystal structure with phage peptide GEEWGPWV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2012-09-25
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	34.803, 37.772, 105.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.708 - 1.802
R-factor	0.1876
Rwork	0.185
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bcx
RMSD bond length	0.006
RMSD bond angle	1.098
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.710	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.100	0.490
Number of reflections	13212
<I/σ(I)>	11.6	2.7
Completeness [%]	97.7	8
Redundancy	6.4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		100 MM MES/IMIDAZOLE PH 6.5, 0.02 M 1,6-HEXANEDIOL, 0.02 M 1-BUTANOL, 0.02 M (RS)-1,2- PROPANEDIOL, 0.02 M 2-PROPANOL, 0.02 M 1,4-BUTANEDIOL, 0.02 M 1,3-PROPANEDIOL, 10% (W/V) PEG 20000 AND 24% (V/V) PEG400 (USING A 2:1 RATIO OF PROTEIN TO MOTHER LIQUOR)