2YMS
Structure and assembly of a b-propeller with nine blades and a new conserved repetitive sequence motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.570, 83.450, 57.370 |
Unit cell angles | 90.00, 118.94, 90.00 |
Refinement procedure
Resolution | 50.206 - 2.101 |
R-factor | 0.1899 |
Rwork | 0.187 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.069 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.450 |
Number of reflections | 21022 | |
<I/σ(I)> | 7.4 | 1.6 |
Completeness [%] | 93.4 | 91.2 |
Redundancy | 2.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |